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	Provedor de dados BABT (1) Electron. J. Biotechnol. (1) Autor Aroca Arcaya,Germán (1) Caballero Valdés,Eduardo (1) Grade,Luciana Carvalho (1) Ida,Elza Iouko (1) Mandarino,José Marcos Gontijo (1) Moreira,Amanda Aleixo (1) Ribeiro,Mara Lúcia Luiz (1) Silva,Josemeyre Bonifácio da (1) Varea,Geni da Silva (1) Wilson Soto,Lorena (1) Mais... Palavra-chave Immobilisation (2) Aglycones (1) Albumin (1) Chitosan (1) Dextran aldehyde (1) Glutaraldehyde (1) Lipase (1) Β-glucosidase soybean (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2014 (1) 2011 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Influence of the pH of glutaraldehyde and the use of dextran aldehyde on the preparation of cross-linked enzyme aggregates (CLEAs) of lipase from Burkholderia cepacia Electron. J. Biotechnol. Caballero Valdés,Eduardo; Wilson Soto,Lorena; Aroca Arcaya,Germán. The preparation of cross-linked enzyme aggregates (CLEAs) of lipase has been a challenge due the low amount of lysine residues that lipases have on their surface. The results show that CLEAs prepared using dextran aldehyde (100-200KDa) have a higher hydrolysis activity and particle size (activities between 3186 ± 21 U/g of CLEA and 4800 ± 30 U/g of CLEA and particle sizes between 52.6 ± 18.7 µm and 126.2 ± 53.5 µm) than CLEAs prepared with glutaraldehyde (0.1 KDa) (activities between 894 ± 16 U/g of CLEA and 2874 ± 20 U/g of CLEA and particle sizes between 21.2 ± 5.1 µm and 83.4 ± 24.9 µm); Thermal stability assays of bioctalysts at 60ºC at pH 7.0 using phosphate buffer 25 mM showed that CLEAs prepared with dextran aldehyde have lower residual... Tipo: Journal article Palavras-chave: Albumin; Dextran aldehyde; Glutaraldehyde; Immobilisation; Lipase. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300010 Soybean β-Glucosidase immobilisated on chitosan beads and its application in soy drink increase the aglycones BABT Grade,Luciana Carvalho; Moreira,Amanda Aleixo; Varea,Geni da Silva; Mandarino,José Marcos Gontijo; Silva,Josemeyre Bonifácio da; Ida,Elza Iouko; Ribeiro,Mara Lúcia Luiz. The objective of this study was to investigate the immobilisation efficiency of soybean β-glucosidase (181.6 U/mL; 23.8 mg protein/mL) on activated chitosan beads. Central Composite Rotational Design (CCDR) 23 was used and the application of immobilised enzyme in commercial soy drink was evaluated. The activation of chitosan beads was achieved with established 2.5% glutaraldehyde, pH 7.5, 8 h incubation time (6 h with agitation and 2 h without agitation) at 37ºC. The highest immobilisation efficiency (%) of soybean β-glucosidase on chitosan beads obtained was 37.74 U/mL and 18.84 mg protein/4 chitosan beads at pH 7.5 and 20 h coupling time of enzyme-matrix (7 h with agitation and 13 h without agitation) at 4ºC. The immobilised enzyme incubated at 50ºC, pH... Tipo: Info:eu-repo/semantics/article Palavras-chave: Β-glucosidase soybean; Immobilisation; Chitosan; Aglycones. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500766 Registros recuperados: 2 Primeira ... 1 ... Última

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